Studies on pituitary adrenocorticotropin. 2. Oxidation–reduction properties of the hormone*

Studies on pituitary adrenocorticotropin. II. Oxidation-reduction properties of the hormone.

Studies on Pituitary Lactogenic Hormone

Previous investigations have shown that the ovine prolactin is composed of a single chain at the N terminus, with threonine as the terminal residue (1). Since the hormone protein has 3 cystine residues (2, 3), it might be anticipated that one or more of these three -S-Sbridges may form a branched chain in the C terminus or along a portion of the polypeptide chain. If this were the case, oxidati...

Studies on Pituitary Lactogenic Hormone

The Tiselius technique of electrophoresis has been used to study the homogeneity and mobility of pituitary lactogenic hormone. The lactogenic preparation employed by us shows only one sharp band in schlieren photographs, indicating a high degree of homogeneity. From the determination of the mobility of the hormone at different hydrogen ion concentrations in acetate and phosphate buffer of ionic...

STUDIES ON PITUITARY LACTt)GEI’tIC HORMONE

In recent publications experiments were described demonstrating the homogeneity of our lactogenic hormone preparations by means of both electrophoretic (1, 2) and solubility (3) criteria. Of pituitary hormones the lactogenic seems to have been the first isolated in chemically pure form. Its molecular weight, however, has not yet been reported, although for many years it has been thought to be c...

Cytokine-dependent gp130 receptor subunit regulates human fetal pituitary adrenocorticotropin hormone and growth hormone secretion.

We have shown recently that leukemia inhibitory factor (LIF) and oncostatin M (OSM), two members of the gp130-dependent cytokine family, stimulate murine proopiomelanocortin (POMC) transcription and adrenocorticotropin hormone (ACTH) secretion. LIF and corticotropin-releasing hormone (CRH) also synergistically induced in vivo ACTH secretion in fetal nonhuman primates. To elucidate the role of t...